A novel 3-D nano-assembly bacteria based biosensor for enhanced detection of heavy metal pollutants

Abstract : Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficult but important task. The current major limitation lies in the poor detection limits of the targeted pollutant's trace concentrations by the available conventional techniques. In order to elaborate a novel "living" self assembled electrochemical 3-D biosensor, the authors propose a new concept to overcome this shortcoming. The advantages of the properties of polyelectrolyte-functionalized NBs (nanobeads) are combined along with the use of non covalently strongly bound micro-organisms. The designed 3-D biosensor is all the more promising as it has showed a significantly improved sensitivity. In fact, the detection limits of the tested heavy metals (cadmium and mercury) were as low as 1.0 × 10-12 mol*L-1 and six to seven orders of magnitude lower than those provided by conventional 2-D biosensors. Furthermore, it is potentially applicable to a wide range of bioreceptor-pollutant detection systems.
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https://hal.archives-ouvertes.fr/hal-00780449
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Submitted on : Thursday, January 24, 2013 - 4:22:49 AM
Last modification on : Thursday, February 7, 2019 - 5:53:17 PM

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  • HAL Id : hal-00780449, version 1

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Mina Souiri, Ibtissem Gammoudi, Laurence Mora, Hafedh Ben Ouada, Thierry Jouenne, et al.. A novel 3-D nano-assembly bacteria based biosensor for enhanced detection of heavy metal pollutants. Journal of Environmental Science and Engineering, 2012, 1 (7A), pp.936-950. ⟨hal-00780449⟩

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