Structure and Charge-State Dependence of the Gas-Phase Ionization Energy of Proteins.

Alexandre Giuliani (1) , Aleksandar R Milosavljević , Konrad Hinsen (1, 2) , Francis Canon , Christophe Nicolas , Matthieu Réfrégiers , Laurent Nahon (1)

1 SSOLEIL - Synchrotron SOLEIL
2 CBM - Centre de biophysique moléculaire

Photoionization of protein ions: The ionization energy of polyprotonated protein cations in the gas phase measured using VUV synchrotron radiation appears to be correlated with the charge state z of the protein and its tertiary structure. A simple electrostatic model accounts for the results and also shows predictive capabilities to derive a mean radius R(m) of the protein ion from the ionization energy, and vice versa.

Mots clés: ionization potentials; mass spectrometry; protein structures

Domaines: Biochimie, Biologie Moléculaire

Alexandre Giuliani, Aleksandar R Milosavljević, Konrad Hinsen, Francis Canon, Christophe Nicolas, et al.. Structure and Charge-State Dependence of the Gas-Phase Ionization Energy of Proteins.. Angewandte Chemie International Edition, 2012, 51, epub ahead of print. ⟨10.1002/anie.201204435⟩. ⟨hal-00727349⟩