Comparison of collision-induced dissociation and electron-induced dissociation of singly charged mononucleotides

Viet Hung Nguyen (1) , Carlos Afonso (1, 2) , Jean-Claude Tabet (1)

1 IPCM - Institut Parisien de Chimie Moléculaire
2 COBRA - Chimie Organique et Bioorganique : Réactivité et Analyse

The dissociation of singly charged mononucleotides by electron induced dissocn. (EID) and sustained off resonance irradiation/collision induced dissociation (SORI-CID) was investigated. The experiments have been carried out on deprotonated mononucleotides monomers. In general the same productions are observed in the EID and SORI-CID spectra but some additional productions have been obtained with EID. It was shown by MS3 experiments that all these addional productions have been produced through consecutive dissociations. of the [M-2H][rad] - radical anion. In addition, H/D exchange and sequential MS3 experiments demonstrated that the m/z 247 fragment ion produced in EID of deprotonated 2'-deoxyguanosine 5'-monophosphate corresponds to the association of the phosphate group and the nucleobase. They were most likely linked by a non-covalent bond that was initially present in the precursor ion.

Domaines: Chimie analytique

Viet Hung Nguyen, Carlos Afonso, Jean-Claude Tabet. Comparison of collision-induced dissociation and electron-induced dissociation of singly charged mononucleotides. International Journal of Mass Spectrometry, 2012, 316-318, pp.140-146. ⟨10.1016/j.ijms.2012.01.015⟩. ⟨hal-00708364⟩