Electrochemical monitoring of Chlorhexidine Digluconate effect on polyelectrolyte immobilized bacteria and kinetic cell adhesion

Abstract : The electrochemical impedance spectroscopy (EIS) technique has been used as a sensitive method to explore the effect of antibacterial molecules on immobilized bacteria and biofilm formation. In this work, we describe the electrochemical spectroscopy as a powerful method to monitor the effect of Chlorhexidine Digluconate (CHX-Dg) on polyelectrolyte immobilized Escherichia coli K12 MG1655 and the kinetics of cell adhesion on gold electrodes. The experimental impedance data were modelised with a Zview program to find the best equivalent electrical circuit and analyse its parameter's properties. Polyelectrolyte multilayer formation on the electrode surface and bacteria immobilization greatly increased the electron-transfer resistance (Ret) and reduced the constant phase element (CPEdl). The effect of CHX-Dg was studied in a 0.5 × 10−4 mmol l−1 to 0.5 mmol l−1 range. The relation between the evolution of Ret and CHX-Dg concentration was found to be negatively correlated. When CHX-Dg was added, the electrochemical monitoring of the bacterial kinetic adhesion showed that the electrode's capacity (CP) variation remained stable, demonstrating that the addition of CHX-Dg in the broth inhibited bacterial adhesion.
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Article dans une revue
Journal of Biotechnology, Elsevier, 2011, 151 (1), pp.114-121. 〈10.1016/j.jbiotec.2010.11.014〉
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https://hal.archives-ouvertes.fr/hal-00703858
Contributeur : Agnès Bussy <>
Soumis le : lundi 4 juin 2012 - 14:37:52
Dernière modification le : jeudi 7 février 2019 - 17:21:01

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Nabil Borghol, Laurence Mora, N. Sakly, Philippe Lejeune, Thierry Jouenne, et al.. Electrochemical monitoring of Chlorhexidine Digluconate effect on polyelectrolyte immobilized bacteria and kinetic cell adhesion. Journal of Biotechnology, Elsevier, 2011, 151 (1), pp.114-121. 〈10.1016/j.jbiotec.2010.11.014〉. 〈hal-00703858〉

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