Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences

Zouhair M. Baccar (1) , David Caballero (2) , Ramon Eritja (3, 4) , Abdelhamid Errachid (5)

1 INRAP - Institut National de Recherche et d'Analyse Physico-Chimique
2 Nanobioengineering Group-IBEC
3 IQAC-CSIC - Institute for Advanced Chemistry of Catalonia
4 IRB Barcelona - Institute for Research on Biomedicine
5 SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014)

DNA testing requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. In this paper, a simple and label-free DNA-biosensor has been investigated based on the detection of DNA hybridization on layered double hydroxide (LDH) nanomaterials with special emphasis on targeting long single stranded DNA sequences. First, the immobilization of a 20 bases long DNA probe on a thin layer of Mg2AlCO3 and Mg3AlCO3 LDH was studied. Then, DNA hybridization reaction was detected by means of Electrochemical Impedance Spectroscopy. The resulting biosensor showed a high sensitivity for the detection of 80 bases long DNA complementary sequences. The dynamic range was 18-270 ng/ml with a detection limit lower than 1.8 ng/ml.

Mots clés: DNA-biosensor; Nanomaterials; Layered double hydroxide; Self-assembly

Domaines: Chimie analytique

Zouhair M. Baccar, David Caballero, Ramon Eritja, Abdelhamid Errachid. Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences. Electrochimica Acta, 2012, 74, pp.123-129. ⟨10.1016/j.electacta.2012.04.031⟩. ⟨hal-00703634⟩