Multiplexed binding determination of seven glycoconjugates for Pseudomonas aeruginosa lectin I (PA-IL) using a DNA-based carbohydrate microarray.

Yann Chevolot (1) , Jing Zhang (2) , Albert Meyer (3) , Alice Goudot (1) , Sonia Rouanet (3) , Sébastien Vidal (4) , Gwladys Pourceau (3) , Jean-Pierre Cloarec (1) , Jean-Pierre Praly (4) , Eliane Souteyrand (1) , Jean-Jacques Vasseur (3) , François Morvan (3)

1 INL - C&N - INL - Chimie et Nanobiotechnologies
2 LMVI - Key Laboratory of Molecular Virology & Immunology
3 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
4 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

The binding of seven multivalent glycoconjugates displaying linear or antenna-like structures and different electronic environments were evaluated towards PA-IL on a DNA-based carbohydrate microarray. The affinity can be modulated by the charge and the topology of the galactosylated derivatives.

Domaines: Chimie organique

Yann Chevolot, Jing Zhang, Albert Meyer, Alice Goudot, Sonia Rouanet, et al.. Multiplexed binding determination of seven glycoconjugates for Pseudomonas aeruginosa lectin I (PA-IL) using a DNA-based carbohydrate microarray.. Chemical Communications, 2011, 47 (31), pp.8826-8. ⟨10.1039/c1cc12428e⟩. ⟨hal-00695908⟩