Abstract : With the convergence of computing and communication, and the expansion of cloud computing, new models and tools are needed to allow users to define, create, and exploit on-demand virtual infras- tructures within wide area distributed environments. Optimally design- ing customized virtual execution-infrastructure and executing them on a physical substrate remains a complex problem. This paper presents the VXDL language, a language for specifying and describing virtual infras- tructures and the HIPerNET framework to manage them. Based on the example of a specific biomedical application and workflow engine, this paper illustrates how VXDL enables to specify different customized vir- tual infrastructures and the HIPerNET framework to execute them on a distributed substrate. The paper presents experiments of the deploy- ment and execution of this application on different virtual infrastructures managed by our HIPerNet system. All the experiments are performed on the Grid'5000 testbed substrate.