Fluorescence correlation spectroscopy near individual gold nanoparticle

Qingyan Wang (1) , Guowei Lu (1) , Lei Hou (1) , Tianyue Zhang (1) , Chunxiong Luo (1) , Hong Yang (1) , Grégory Barbillon (2) , Franck Lei (3) , Christophe a Marquette (4, 5) , Pascal Perriat (6) , Olivier Tillement (7) , Stéphane Roux (8, 7) , Qi Ouyang (1) , Qihuang Gong (1)

1 State Key Laboratory of Pathogen and Biosecurity
2 Laboratoire Charles Fabry de l'Institut d'Optique / Biophotonique
3 Unite Medicament Dynam Intracellulaire Architectu, CNRS, UFR Pharm
4 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
5 Axo S - AXO Science
6 MATEIS - Matériaux, ingénierie et science [Villeurbanne]
7 LPCML - Laboratoire de Physico-Chimie des Matériaux Luminescents
8 Phys-ENS - Laboratoire de Physique de l'ENS Lyon

Dynamic behavior of fluorescent molecules near an individual gold nanoparticle is investigated experimentally by fluorescence correlation spectroscopy method. The gold particle that acts as an optical nano-antenna presents significant near-field volume reduction. The single molecule diffusion behavior is clearly observed within a reduced near-field volume due to a highly localized field enhancement. The near-field volume and fluorescence enhancement are polarization and concentration dependent and strongly depend on the properties of the gold nanoparticle. A simple approximated model is developed to fit the FCS autocorrelation curves. In principle, the single molecule analysis within the near-field volume of nanostructures could be applied to the analysis of biological membranes and intracellular processes. (C) 2011 Elsevier B.V. All rights reserved.

Mots clés: SINGLE-MOLECULE FLUORESCENCE; PARTICLE; ENHANCEMENT; VOLUME

Domaines: Biochimie, Biologie Moléculaire

Qingyan Wang, Guowei Lu, Lei Hou, Tianyue Zhang, Chunxiong Luo, et al.. Fluorescence correlation spectroscopy near individual gold nanoparticle. Chemical Physics Letters, 2011, 503 (4-6), pp.256-261. ⟨10.1016/j.cplett.2011.01.005⟩. ⟨hal-00877001⟩