JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.

Marie-Laure Arcangeli (1) , Vincent Frontera (1) , Florence Bardin (2, 1) , Elodie Obrados (1) , Susanne Adams (3) , Christian Chabannon (4) , Claudine Schiff (5) , Stephane J C Mancini (5) , Ralf H Adams (3) , Michel Aurrand-Lions (1)

1 CRCM / U891 Inserm - Centre de Recherche en Cancérologie de Marseille
2 Thérapie cellulaire et génique
3 Department of Tissue Morphogenesis [Münster]
4 Centre de Ressources Biologiques en Oncologie
5 CIML - Centre d'Immunologie de Marseille - Luminy

In adult mammals, hematopoietic stem cells (HSCs) reside in the bone marrow (BM) and are maintained in a quiescent and undifferentiated state through adhesive interactions with specialized microenvironmental niches. Although junctional adhesion molecule-C (JAM-C) is expressed by HSCs, its function in adult hematopoiesis remains elusive. Here, we show that HSCs adhere to JAM-B expressed by BM stromal cells in a JAM-C dependent manner. The interaction regulates the interplay between HSCs and BM stromal cells as illustrated by the decreased pool of quiescent HSCs observed in jam-b deficient mice. We further show that this is probably because of alterations of BM stromal compartments and changes in SDF-1α BM content in jam-b(-/-) mice, suggesting that JAM-B is an active player in the maintenance of the BM stromal microenvironment.

Domaines: Immunologie

Marie-Laure Arcangeli, Vincent Frontera, Florence Bardin, Elodie Obrados, Susanne Adams, et al.. JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.. Blood, 2011, 118 (17), pp.4609-19. ⟨10.1182/blood-2010-12-323972⟩. ⟨hal-00667753⟩