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Lipid packing variations induced by pH in cardiolipin-containing bilayers: The driving force for the cristae-like shape instability

Abstract : Cardiolipin is a four-tailed acidic lipid found predominantly within the inner membrane of mitochondria, and is thought to be a key component in determining inner membrane properties and potential. Thus, cardiolipin may be involved in the dynamics of the inner membrane characteristic invaginations (named cristae) that protrude into the matrix space. Laurdan fluorescence and ζ-potential measurements show that under pH decrease, membrane surface charge decreases, but that significant modification of the lipid packing is observed only for CL-containing membranes. Our giant unilamellar vesicle experiments also indicate that cristae-like morphologies are only observed for CL-containing lipid membranes. Taken together, these results highlight the fact that only a strong modulation of the lipid packing of the exposed monolayer leads to membrane shape instability and suggest that mitochondrial lipids, in particular the cardiolipin, play a specific role under pH modulation in inner mitochondrial membrane morphology and dynamics.
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https://hal.archives-ouvertes.fr/hal-00658860
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Submitted on : Wednesday, January 11, 2012 - 2:52:03 PM
Last modification on : Friday, September 24, 2021 - 3:35:59 AM
Long-term archiving on: : Thursday, April 12, 2012 - 2:36:02 AM

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Nada Khalifat, Jean-Baptiste Fournier, Miglena Angelova, Nicolas Puff. Lipid packing variations induced by pH in cardiolipin-containing bilayers: The driving force for the cristae-like shape instability. Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2011, 1808 (11), pp.2724-2733. ⟨10.1016/j.bbamem.2011.07.013⟩. ⟨hal-00658860⟩

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