In situ nanochemical imaging of label-free drugs: a case study of antimalarials in Plasmodium falciparum-infected erythrocytes.

Faustine Dubar (1) , Sylvain Bohic (2) , Christian Slomianny , Jean-Charles Morin (3) , Patrick Thomas (4) , H. Kalamou (5) , Yann Guérardel (6) , Peter Cloetens (7) , Jamal Khalife (8) , Christophe Biot (6)

1 UCCS - Unité de Catalyse et Chimie du Solide - UMR 8181
2 INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
3 IPL Santé Environnement Durable
4 IPL Santé Environnement Durable
5 CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204
6 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576
7 ESRF - European Synchrotron Radiation Facility
8 Schistosomiase, paludisme et inflammation

We report here for the first time the in vitro localization of unlabeled antimalarial drugs with high spatial resolution. This strategy further enhances our understanding of the action mechanisms of antimalarial drugs. Our approach may be applied to a wide range of domains where quantitative chemical imaging of drugs at the sub-cellular level appears critical.

Domaines: Biochimie [q-bio.BM]

Faustine Dubar, Sylvain Bohic, Christian Slomianny, Jean-Charles Morin, Patrick Thomas, et al.. In situ nanochemical imaging of label-free drugs: a case study of antimalarials in Plasmodium falciparum-infected erythrocytes.. Chemical Communications, 2012, 48 (6), pp.910-912. ⟨10.1039/C1CC16211J⟩. ⟨hal-00652186⟩