Biochemical characterization of Extracellular Polymeric Substances extracted from an intertidal mudflat using a cation exchange resin.

Abstract : The biochemical characterization of Extracellular Polymeric Substances (EPS) excreted in a European intertidal mudflat (Marennes-Oléron Bay) was performed. Experiments were carried out for the first time in situ, by using an improved extraction recently developed. This innovative procedure, using a cation exchange resin (Dowex), allows separating precisely different fractions of EPS, especially pure bound EPS. Moreover, it avoids the contamination of EPS fractions by residual and intracellular polymers, enabling to properly estimate polymeric contents in each fraction. The results were partly similar to conventional results described in the literature and the amount of colloidal carbohydrates (146µg/g of dry sediment) extracted by the Dowex method fitted well with different EPS estimation in European mudflats. Colloidal carbohydrates were essentially composed of glucose (>50%), a carbon source rapidly consumed by the various communities in the sediment. Pure bound carbohydrates were composed of specific carbohydrates (28% rhamnose, 22% xylose). Residual fractions, considered as containing some refractory bound EPS and mostly other internal polymeric substances, presented a more varied composition rich in carbohydrates: galacturonic acid (20%), mannose (19.5%), glucose (19%), arabinose (15%), xylose (8%), galactose (7%).
Document type :
Journal articles
Complete list of metadatas

Cited literature [1 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00652078
Contributor : Marianne Graber <>
Submitted on : Thursday, December 15, 2011 - 11:47:47 AM
Last modification on : Tuesday, February 25, 2020 - 4:22:06 PM
Long-term archiving on: Friday, March 16, 2012 - 2:21:54 AM

Files

Pub_Biochem_Syst_Ecol_final_GP...
Files produced by the author(s)

Identifiers

  • HAL Id : hal-00652078, version 1

Citation

Guillaume Pierre, Marianne Graber, Francis Orvain, Christine Dupuy, Thierry Maugard. Biochemical characterization of Extracellular Polymeric Substances extracted from an intertidal mudflat using a cation exchange resin.. Biochemical Systematics and Ecology, Elsevier, 2010, 38, pp.917-923. ⟨hal-00652078⟩

Share

Metrics

Record views

329

Files downloads

878