Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica.

Feten Abbassi (1) , Bruno Oury , Thierry Blasco , Denis Sereno (2) , Gérard Bolbach (3) , Pierre Nicolas (4) , Khaled Hani (5) , Mohamed Amiche (6) , Ali Ladram (1)

1 PBSF - Protéines : biochimie structurale et fonctionnelle
2 IRD - UR008 Pathogénie des Trypanosomatidés
3 UPMC - Université Pierre et Marie Curie - Paris 6
4 IMTSSA - Institut de Médecine Tropicale du Service de Santé des Armées
5 Laboratory of Biochemistry
6 IJM (UMR_7592) - Institut Jacques Monod

Temporins are small antimicrobial peptides isolated from North American and Eurasian ranid frogs that are particularly active against Gram-positive bacteria. To date, no temporins have been characterized from North African frog species. We isolated three novel members of the temporin family, named temporin-1Sa (FLSGIVGMLGKLF(amide)), -1Sb (FLPIVTNLLSGLL(amide)), and -1Sc (FLSHIAGFLSNLF(amide)), from the skin of the Sahara frog Pelophylax (Rana) saharica originating from Tunisia. These temporins were identified by a combined mass spectrometry/molecular cloning approach. Temporin-1Sa was found to be highly active against Gram-positive and Gram-negative bacteria, yeasts and fungi (MIC=2-30 microM). To our knowledge, this is the first 13-residue member of the temporin family with a net charge of +2 that shows such broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC=10 microM) and Pseudomonas aeruginosa (MIC=31 microM). Moreover, temporin-1Sa displays significant antiparasitic activity (IC50 approximately 20 microM) against the promastigote and amastigote stages of Leishmania infantum.

Domaines: Chimie organique

Feten Abbassi, Bruno Oury, Thierry Blasco, Denis Sereno, Gérard Bolbach, et al.. Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica.. Peptides, 2008, 29 ((9)), pp.1526-33. ⟨10.1016/j.peptides.2008.05.008⟩. ⟨hal-00649680⟩