Implementation of electrochemiluminescence microanalysis in PCB technology.

Patrick Pittet (1) , Guo-Neng Lu , Jean-Marc Galvan (1) , Rosaria Ferrigno (2) , Khaled Stephan , Loïc J Blum (3) , Béatrice D Leca-Bouvier (3)

1 INL - CSH - INL - Conception de Systèmes Hétérogènes
2 INL - LOCI - INL - Lab-On-Chip et Instrumentation
3 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

We present an instrumental development to implement electrochemiluminescence (ECL) microanalysis using printed circuit board (PCB) technology. PCB gold macro-(10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.

Domaines: Biochimie [q-bio.BM]

Patrick Pittet, Guo-Neng Lu, Jean-Marc Galvan, Rosaria Ferrigno, Khaled Stephan, et al.. Implementation of electrochemiluminescence microanalysis in PCB technology.. Conference proceedings : .. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2007, 2007, pp.2944-7. ⟨10.1109/IEMBS.2007.4352946⟩. ⟨hal-00631226⟩