Transverse chemical interface detection with coherent anti-Stokes Raman scattering microscopy

Sophie Brustlein (1) , David Gachet (2) , Franck Billard (3, 4) , Herve Rigneault (1)

1 MOSAIC - MOSAIC
2 CINaM - Centre Interdisciplinaire de Nanoscience de Marseille
3 FRESNEL - Institut FRESNEL
4 ICB - Laboratoire Interdisciplinaire Carnot de Bourgogne

Transverse "chemical" interfaces are revealed with a conventional two beam narrowband coherent anti-Stokes Raman scattering microscopy setup in a collinear configuration. The exciting "pump" and "Stokes" beams are focused on the sample in two opposite directions. The subtraction of the two generated anti-Stokes signals gives rise to a signal that is directly proportional to the pure Raman spectrum of the resonant medium. This property is used to highlight an interface between glass and N,N-dimethylformamide (DMF) and recover the pure Raman spectrum of DMF around its 1408 cm−1 vibrational band.

Domaines: Optique / photonique

Sophie Brustlein, David Gachet, Franck Billard, Herve Rigneault. Transverse chemical interface detection with coherent anti-Stokes Raman scattering microscopy. Journal of Biomedical Optics, 2011, 16, pp.086006. ⟨hal-00613889⟩