Development of 55 novel polymorphic microsatellite loci for the critically endangered L. (Actinopterygii: Perciformes: Percidae) and cross-species amplification in five other percids

Vincent Dubut (1) , Rémi Grenier (1) , Emese Meglécz (1) , Rémi Chappaz (1) , Caroline Costedoat (1) , Delphine Danancher (2, 3) , Stéphane Descloux (4) , Thibaut Malausa (5) , Jean-François Martin (6) , Nicolas Pech (1) , André Gilles (1)

1 IMEP - Institut Méditerranéen d'Ecologie et de Paléoécologie
2 Conservatoire Rhône-Alpes des Espaces Naturels
3 UCBL - Université Claude Bernard Lyon 1
4 Electricité de France, Service Environnement et Société, Centre d'Ingénierie Hydraulique
5 ISA - Institut Sophia Agrobiotech
6 UMR CBGP - Centre de Biologie pour la Gestion des Populations

By combining biotin-enrichment protocol and next generation pyrosequencing, through 454 GS-FLX Titanium technology, 55 polymorphic microsatellites loci with perfect motif were isolated from the Rhone streber (), a critically endangered European fish species. Eight multiplex PCR kits were optimised in order to genotype a total of 58 polymorphic loci, including three previously published loci. The level of genetic diversity was assessed for 68 , 30 , 33 and four individuals. Amplification success was also assessed on and using single individuals. These markers will be useful to investigate the population structure of the highly fragmented Rhone streber. They represent a powerful tool for conservation issues and evolutionary approaches of this endemic species. Moreover, part of our markers demonstrated applicability to other percid species, allowing for potential applications to fisheries and aquaculture management.

Mots clés: Percidae; STRs; Enriched library; Pyrosequencing; QDD; Conservation genetics

Domaines: Sciences du Vivant [q-bio]

Vincent Dubut, Rémi Grenier, Emese Meglécz, Rémi Chappaz, Caroline Costedoat, et al.. Development of 55 novel polymorphic microsatellite loci for the critically endangered L. (Actinopterygii: Perciformes: Percidae) and cross-species amplification in five other percids. European Journal of Wildlife Research, 2010, 56 (6), pp.931-938. ⟨10.1007/s10344-010-0421-x⟩. ⟨hal-00609749⟩