A Pyrophosphate-Responsive Gadolinium(III) MRI Contrast Agent

Abstract : This study shows that the relaxivity and optical properties of functionalised lanthanide-DTPA-bis-amide complexes (lanthanide = Gd3+ and Eu3+, DTPA = diethylene triamine pentaacetic acid) can be successfully modulated by addition of specific anions, without direct Ln(3+)/anion coordination. Zinc(II)-dipicolylamine moieties, which are known to bind strongly to phosphates, were introduced in the amide "arms" of these ligands, and the interaction of the resulting Gd-Zn-2 complexes with a range of anions was screened by using indicator displacement assays (IDAs). Considerable selectivity for polyphosphorylated species (such as pyrophosphate and adenosine-5'-triphosphate (ATP)) over a range of other anions (including monophosphorylated anions) was apparent. In addition, we show that pyrophosphate modulates the relaxivity of the gadolinium(III) complex, this modulation being sufficiently large to be observed in imaging experiments. To establish the binding mode of the pyrophosphate and gain insight into the origin of the relaxometric modulation, a series of studies including UV/Vis and emission spectroscopy, luminescence lifetime measurements in H2O and D2O, O-17 and P-31 NMR spectroscopy and nuclear magnetic resonance dispersion (NMRD) studies were carried out.
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Submitted on : Tuesday, July 12, 2011 - 3:47:10 PM
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Andrew J. Surman, Celia S. Bonnet, Mark P. Lowe, Gavin D. Kenny, Jimmy D. Bell, et al.. A Pyrophosphate-Responsive Gadolinium(III) MRI Contrast Agent. Chemistry - A European Journal, Wiley-VCH Verlag, 2011, 17 (1), pp.223-230. ⟨10.1002/chem.201001397⟩. ⟨hal-00608253⟩



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