SERS detection of biomolecules using lithographied nanoparticles towards a reproducible SERS biosensor

Catalina David (1) , Nicolas Guillot (1) , Hong Shen (2) , Timothée Toury (2) , Marc Lamy de La Chapelle (1)

1 CSPBAT - Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques
2 LNIO - Laboratoire de Nanotechnologie et d'Instrumentation Optique

In this paper we highlight the accurate spectral detection of bovine serum albumin and ribonuclease-A using a Surface-Enhanced Raman Scattering (SERS) substrate based on gold nanocylinders obtained by Electron-Beam Lithography (EBL). The nanocylinders have diameter from 100 to 180 nm with a gap of 200 nm. We demonstrate that optimizing the size and the shape of the lithographied gold nanocylinders, we can obtain SERS spectra of proteins at low concentration. This SERS study enabled us to estimate high enhancement factors (105 for BSA and 107 for RNase-A) of important bands in the protein Raman spectrum measured for 1mM concentration. We demonstrate that to reach the highest enhancement it is necessary to optimize the SERS signal and that the main parameter of optimization is the LSPR position. The LSPR have to be suitably located between the laser excitation wavelength which is 632.8 nm and the position of the considered Raman band. Our study underlines the efficiency of gold nanocylinders arrays in spectral detection of proteins.

Mots clés: IMMUNOASSAY; FILMS; SILVER; ALBUMIN; ENHANCED RAMAN-SCATTERING; PLASMON RESONANCE; SURFACE; SPECTROSCOPY; ADSORBATES; MONOLAYERS

Domaines: Optique [physics.optics]; Optique / photonique

Catalina David, Nicolas Guillot, Hong Shen, Timothée Toury, Marc Lamy de La Chapelle. SERS detection of biomolecules using lithographied nanoparticles towards a reproducible SERS biosensor. Nanotechnology, 2010, 21 (47), ⟨10.1088/0957-4484/21/47/475501⟩. ⟨hal-00629337⟩