Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method.

Catherine Aude-Garcia (1) , Véronique Collin-Faure (1) , Sylvie Luche (1) , Thierry Rabilloud (1)

1 BBSI - Biochimie et biophysique des systèmes intégrés

Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost-effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method.

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Catherine Aude-Garcia, Véronique Collin-Faure, Sylvie Luche, Thierry Rabilloud. Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method.. Proteomics, 2011, 11 (2), pp.324-8. ⟨10.1002/pmic.201000370⟩. ⟨hal-00565511⟩