Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors

Abstract : A very-long-chain (C22) organosilicon self-assembled monolayer (SAM) with terminal glycidyl groups was used to immobilize antibodies on the silica waveguide of a Love mode surface acoustic wave (SAW) immunosensor. This route enabled us to specifically detect, in real time, living Escherichia coli bacteria by using monoclonal anti-E. coli antibodies, leading to a marked sensor response in liquid aqueous medium. Conversely, no bacteria detection could be observed by this direct method upon functionalization of the waveguide surface by a (3-glycidoxypropyl) trimethoxysilane (GPTS) monolayer. This improvement was attributed to a surface smoothing effect resulting from the very-long-chain SAM and a better accessibility of the surface epoxide groups.
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https://hal.archives-ouvertes.fr/hal-00548745
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Submitted on : Monday, December 20, 2010 - 12:28:25 PM
Last modification on : Tuesday, February 27, 2018 - 11:18:10 PM

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Duy Hai Dinh, Emilie Pascal, Luc Vellutini, Bernard Bennetau, Dominique Rebière, et al.. Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors. Sensors and Actuators B: Chemical, Elsevier, 2010, 146 (1), pp.289-296. ⟨10.1016/j.snb.2010.02.008⟩. ⟨hal-00548745⟩

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