Uptake of Functionalized Mesoporous Silica Nanoparticles by Human Cancer Cells

Abstract : Mesoporous silica nanoparticles (MSN) were functionalised by aminofluorescein (AMF) with diethylenetriaminepentaacetic acid spacer molecules which provide free carboxylic groups for binding cell-specific ligands such as folate. AMF allowed the exploration of cellular uptake by HeLa cells using confocal microscopy and flow cytometry. The functionalized nanoparticles (MSN-AMF) penetrated efficiently into HeLa cell cytoplasm through a clathrin dependent endocytosis mechanism. The number of endocytosed MSN-AMF was enhanced when using folate as a targeting molecule. Uptake kinetics revealed that most of MSN-AMF were internalized within 4 h of incubation. Moreover, we found that MSN-AMF were capable of escaping the acidic endolysosomal vesicles of HeLa cells. Cytotoxicity studies suggested that these nanoparticles are non-toxic to HeLa cells up to a dose level of 50 mu g/ml.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00529333
Contributor : Isabelle Frapart <>
Submitted on : Monday, October 25, 2010 - 2:17:10 PM
Last modification on : Friday, May 24, 2019 - 11:36:07 AM

Identifiers

Collections

Citation

Matthieu Fisichella, Hinda Dabboue, Sanjib Bhattacharyya, Gerald Lelong, Marie-Louise Saboungi, et al.. Uptake of Functionalized Mesoporous Silica Nanoparticles by Human Cancer Cells. Journal of Nanoscience and Nanotechnology, American Scientific Publishers, 2010, 10 (4), pp.314-2324. ⟨10.1166/jnn.2010.1917⟩. ⟨hal-00529333⟩

Share

Metrics

Record views

276