Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages.
Résumé
The microsporidian Encephalitozoon cuniculi is a unicellular obligate intracellular parasite considered as an emerging opportunistic human pathogen. The differentiation phase of its life cycle leads to the formation of stress-resistant spores. The E. cuniculi genome (2.9 Mbp) having been sequenced, we undertook a descriptive proteomic study of a spore-rich cell population isolated from culture supernatants. A combination of 2-DE and 2-DE-free techniques was applied to whole-cell protein extracts. Protein identification was performed using an automated MALDI-TOF-MS platform and a nanoLC-MS/MS instrument. A reference 2-DE map of about 350 major spots with multiple isoforms was obtained, and for the first time in microsporidia, a large set of unique proteins (177) including proteins with unknown function in a proportion of 25.6% was identified. The data are mainly discussed with reference to secretion and spore structural features, energy and carbohydrate metabolism, cell cycle control and parasite survival in the environment.
Mots clés
Amino Acids
Animals
Dogs
Electrophoresis
Gel
Two-Dimensional
Encephalitozoon cuniculi
Encephalitozoonosis
Humans
Immunohistochemistry
Mass Spectrometry
Peptide Mapping
Protein Interaction Mapping
Proteome
Sequence Analysis
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Spores
Protozoan
Trypsin