Spectroscopic ultrahigh-resolution full-field optical coherence microscopy

Arnaud Dubois (1) , Julien Moreau (2) , Claude Boccara (3)

1 Laboratoire Charles Fabry de l'Institut d'Optique / Elsa - bio
2 Laboratoire Charles Fabry de l'Institut d'Optique / Macsybio - bio
3 LSLP - Laboratoire de Spectroscopie en Lumière Polarisée

We have developed a full-field optical coherence microscopy system providing spectroscopic information with ultra-high spatial resolution in addition to intensity-based images. Local measurements of the shift of the backscattered light spectrum are achieved by short-time Fourier analysis of a stack of en-face interferometric images acquired with a Linnik-type microscope. The intensity images are calculated using a phase-shifting method. Using a halogen lamp as illumination source enables us to achieve spectroscopic imaging over a wavelength range from 600 to 900 nm and to achieve axial resolution of ~ 0.8 µm in tissues. Potential applications in the biomedical field are demonstrated by imaging an ex-vivo Xenopus Laevis tadpole.

Domaines: Optique [physics.optics]

Arnaud Dubois, Julien Moreau, Claude Boccara. Spectroscopic ultrahigh-resolution full-field optical coherence microscopy. Optics Express, 2008, 16 (21), pp.17082. ⟨hal-00520533⟩