Reduced photobleaching of BODIPY-FL

David Dulin (1) , Antoine Le Gall (1) , Karen Perronet (1) , Nicolas Soler (2) , Dominique Fourmy (2) , Satoko Yoshizawa (2) , Philippe Bouyer (3) , Nathalie Westbrook (1)

1 Laboratoire Charles Fabry de l'Institut d'Optique / Otique atomique - bio
2 ICSN - Institut de Chimie des Substances Naturelles
3 laboratoire Charles Fabry de l'Institut d'Optique / Optique atomique

We present a study of the photophysical properties of the BODIPY-FL fluorescent dye at the single molecule scale using total internal reflexion fluorescence microscopy. We characterized the saturation intensity and the maximum number of fluorescence photons for single BODIPY-FL in a usual buffer. We then studied the influence of the buffer depletion in oxygen and the addition of redox agents on the brightness and the lifetime before photobleaching of the BODIPY-FL. The great improvement of these two factors in such a medium allows the use of BODIPY-FL in single-molecule biophysics experiments.

Domaines: Biophysique

David Dulin, Antoine Le Gall, Karen Perronet, Nicolas Soler, Dominique Fourmy, et al.. Reduced photobleaching of BODIPY-FL. HBSM, 2009, Palm Cove, Australia. pp.1563-1567. ⟨hal-00520024⟩