Quantitative Single-Molecule Detection at Ultralow Concentrations.

Philippe Haas (1) , Patrick Then , Andreas Wild (1) , Wilfried Grange (2) , Sylvain Zorman (2) , Martin Hegner , Michel Calame , Ueli Aebi , Josef Flammer , Bert Hecht

1 National Center of Competence for Research in Nanoscale Science (NCCR)
2 IJM (UMR_7592) - Institut Jacques Monod

The applicability of single-molecule fluorescence assays in liquids is limited by diffusion to concentrations in the low picomolar range. Here, we demonstrate quantitative single-molecule detection at attomolar concentrations within 1 min by excitation and detection of fluorescence through a single-mode optical fiber in presence of turbulent flow. The combination of high detectability and short measurement times promises applications in ultrasensitive assays, sensors, and point-of-care medical diagnostics.

Domaines: Chimie analytique

Philippe Haas, Patrick Then, Andreas Wild, Wilfried Grange, Sylvain Zorman, et al.. Quantitative Single-Molecule Detection at Ultralow Concentrations.. Analytical Chemistry, 2010, epub ahead of print. ⟨10.1021/ac100779c⟩. ⟨hal-00505256⟩