Celeribacter neptunius gen. nov. sp. nov., a new member of Alphaproteobacteria.
Résumé
A whitish Gram-negative, motile, aerobic bacterium designated H 14T was isolated from seawater collected in Port Phillip Bay, Melbourne, at St. Kilda beach. Analysis of 16S rRNA gene sequences revealed that the organism belonged to the Roseobacter lineage of the class Alphaproteobacteria forming a distinct evolutionary lineage on the genus level. Strain H 14T was weakly related to Nautella, Ruegeria and Pseudoruegeria (family Rhodobacteraceae). Strain H 14T did not degrade gelatin, casein, chitin, agar and starch, did not produce any carotenoids, did not have Bacteriochlorophyll a, and had limited ability to utilise carbon sources. Strain H 14T grew at 1-8% of NaCl with the temperature range of 5-35 degrees C. Phosphatidylglycerol was the major phospholipid (90%); phosphatidylcholine (7.9%) and phosphatidylethanolamine (2%), were present in minor quantities. The predominant fatty acids were C18:0 (3.8%), C18:1omega9 (5.1%) and C18:1omega7 (82.4%). The DNA base composition for strain H 14T was 59.1 mol% G+C. Based on the results of physiological, biochemical, chemotaxonomic and phylogenetic investigations, new genus Celeribacter gen. nov., with a new species Celeribacter neptunius sp. nov. is proposed. The type strain is H 14T (= KMM 6012T = CIP 109922T).