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Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy.

Abstract : This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environments. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cell environments.
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https://hal.archives-ouvertes.fr/hal-00477175
Contributor : Martine Bondidier <>
Submitted on : Wednesday, April 28, 2010 - 12:10:34 PM
Last modification on : Saturday, June 19, 2021 - 4:17:46 AM

Identifiers

  • HAL Id : hal-00477175, version 1
  • PUBMED : 20389334

Citation

N. Warnasooriya, F. Joud, P. Bun, G. Tessier, M. Coppey-Moisan, et al.. Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy.. Opt Express, 2010, 18 (4), pp.3264-73. ⟨hal-00477175⟩

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