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Journal articles
Cryo-electron tomography of nanoparticle transmigration into liposome.
Olivier Le Bihan
1
Pierre Bonnafous
1
Laszlo Marak
2
Thomas Bickel
3
Sylvain Trépout
1
Stéphane Mornet
4
Felix de Haas
5
Hugues Talbot
6
Jean-Christophe Taveau
1
Olivier Lambert
7
Olivier Le Bihan 1
Author
Pierre Bonnafous 1
Author
Laszlo Marak 2
Author
Thomas Bickel 3
Mail :
Author IdHAL : thomas-bickel ResearcherId : http://www.researcherid.com/rid/B-6434-2008 ORCID : https://orcid.org/0000-0003-0987-7629
Sylvain Trépout 1
Author
Stéphane Mornet 4
Mail :
Author IdHAL : stephane-mornet
Felix de Haas 5
Author
Hugues Talbot 6
Mail :
Author IdHAL : hugues-talbot arXiv : https://arxiv.org/a/hugues_talbot ORCID : https://orcid.org/0000-0002-2179-3498
Jean-Christophe Taveau 1
Author
Olivier Lambert 7
Author
1
CBMN - Chimie et Biologie des Membranes et des Nanoobjets (IECB 2, rue Robert Escarpit 33607 PESSAC CEDEX - France)
- Université Sciences et Technologies - Bordeaux 1 (351 cours de la Libération - 33405 Talence cedex - France)
- ENITAB - École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (France)
- CNRS - Centre National de la Recherche Scientifique : UMR5248 (France)
2
ESYCOM - Electronique, Systèmes de communication et Microsystèmes (Université de Paris-Est - Marne-la-Vallée, Cité Descartes, Bâtiment Copernic, 5 bd Descartes, 77454 Marne-la-Vallée Cedex 2 - France)
- CNAM - Conservatoire National des Arts et Métiers [CNAM] : EA2552 (France)
- UPEM - Université Paris-Est Marne-la-Vallée : EA2552 (5 boulevard Descartes - Champs-sur-Marne - 77454 Marne-la-Vallée Cedex 2 - France)
- ESIEE Paris (2 boulevard Blaise Pascal Cité Descartes BP 99 93162 Noisy-le-Grand Cedex - France)
3
CPMOH - Centre de physique moléculaire optique et hertzienne (Bât. Recherche Physique 351, Cours de la Libération 33405 TALENCE CEDEX - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5798 (France)
- Université Sciences et Technologies - Bordeaux 1 (351 cours de la Libération - 33405 Talence cedex - France)
4
ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux (87 Av du Dr A. Schweitzer 33608 PESSAC CEDEX - France)
- UB - Université de Bordeaux (35, place Pey Berland - 33076 Bordeaux - France)
- CNRS - Centre National de la Recherche Scientifique : UPR9048 (France)
5
FEI Electron Optics (B. V. Achtseweg Noord 5, 5651 GG Eindhoven - Netherlands)
- FEI Electron Optics (France)
7
IECB - Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (2 rue Robert Escarpit, F-33607 Pessac - France)
- Université Sciences et Technologies - Bordeaux 1 (351 cours de la Libération - 33405 Talence cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5471 (France)
Abstract : Nanoparticle transport across cell membrane plays a crucial role in the development of drug delivery systems as well as in the toxicity response induced by nanoparticles. As hydrophilic nanoparticles interact with lipid membranes and are able to induce membrane perturbations, hypothetic mechanisms based on membrane curvature or hole formation have been proposed for activating their transmigration. We report on the transport of hydrophilic silica nanoparticles into large unilamellar neutral DOPC liposomes via an internalization process. The strong adhesive interactions of lipid membrane onto the silica nanoparticle triggered liposome deformation until the formation of a curved neck. Then the rupture of this membrane neck led to the complete engulfment of the nanoparticle. Using cryo-electron tomography we determined 3D architectures of intermediate steps of this process unveiling internalized silica nanoparticles surrounded by a supported lipid bilayer. This engulfing process was achieved for a large range of particle size (from 30 to 200 nm in diameter). These original data provide interesting highlights for nanoparticle transmigration and could be applied to biotechnology development.
Document type :
Journal articles
Complete list of metadatas
https://hal.archives-ouvertes.fr/hal-00436243
Contributor : Stéphane Toulin <>
Submitted on : Thursday, November 26, 2009 - 10:59:37 AM
Last modification on : Thursday, July 30, 2020 - 3:06:33 AM
Contributor : Stéphane Toulin <>
Submitted on : Thursday, November 26, 2009 - 10:59:37 AM
Last modification on : Thursday, July 30, 2020 - 3:06:33 AM
Identifiers
- HAL Id : hal-00436243, version 1
- DOI : 10.1016/j.jsb.2009.07.006
- PUBMED : 19596070
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Citation
Olivier Le Bihan, Pierre Bonnafous, Laszlo Marak, Thomas Bickel, Sylvain Trépout, et al.. Cryo-electron tomography of nanoparticle transmigration into liposome.. Journal of Structural Biology, Elsevier, 2009, 168 (3), pp.419-25. ⟨10.1016/j.jsb.2009.07.006⟩. ⟨hal-00436243⟩
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