Detection of Chlamydia trachomatis by use of polymerase chain reaction

G. Lucotte (1) , M. C. Petit (1, 2) , M. H. François (1) , S. Réveilleau (1)

1 Laboratoires Burckel
2 CBM - Centre de biophysique moléculaire

A polymerase chain reaction (PCR) assay was developed for detection of Chlamydia trachomatis DNA. One primer set was used, from the published sequence of the common C. trachomatis plasmid. Detection of amplified sequences was carried out by agarose gel electrophoresis. Analysis of 106 clinical samples tested by cell culture and PCR showed a sensitivity of 100% when PCR was compared with cell culture.

Domaines: Ingénierie biomédicale

G. Lucotte, M. C. Petit, M. H. François, S. Réveilleau. Detection of Chlamydia trachomatis by use of polymerase chain reaction. Molecular and Cellular Probes, 1992, 6 (2), pp.89-92. ⟨10.1016/0890-8508(92)90051-X⟩. ⟨hal-00433188⟩