Skip to Main content Skip to Navigation
Journal articles

Use of PCR-Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene-Based Identification of Nocardia Species

Abstract : Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Our results confirm that hsp65 PRA must no longer be used for Nocardia species identification, as many species have the same restriction pattern. We then compared sequencing-based strategies using an hsp65 database and a 16S rRNA database and found that the hsp65 region contained sufficient polymorphisms for comprehensive Nocardia species identification.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-00428029
Contributor : Stéphane Delmotte <>
Submitted on : Wednesday, October 28, 2009 - 5:19:17 PM
Last modification on : Wednesday, August 25, 2021 - 12:17:45 PM

Links full text

Identifiers

Collections

Citation

V. Rodriguez-Nava, A. Couble, G. Devulder, Jean-Pierre Flandrois, P. Boiron, et al.. Use of PCR-Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene-Based Identification of Nocardia Species. Journal of Clinical Microbiology, American Society for Microbiology, 2006, 44 (2), pp.536-546. ⟨10.1128/JCM.44.2.536-546.2006⟩. ⟨hal-00428029⟩

Share

Metrics

Record views

741