Skip to Main content Skip to Navigation
Journal articles

Homology-dependent methylation in primate repetitive DNA.

J. Meunier A. Khelifi V. Navratil 1 L. Duret 2 
1 Baobab
PEGASE - Département PEGASE [LBBE]
Abstract : In mammals, several studies have suggested that levels of methylation are higher in repetitive DNA than in nonrepetitive DNA, possibly reflecting a genome-wide defense mechanism against deleterious effects associated with transposable elements (TEs). To analyze the determinants of methylation patterns in primate repetitive DNA, we took advantage of the fact that the methylation rate in the germ line is reflected by the transition rate at CpG sites. We assessed the variability of CpG substitution rates in nonrepetitive DNA and in various TE and retropseudogene families. We show that, unlike other substitution rates, the rate of transition at CpG sites is significantly (37%) higher in repetitive DNA than in nonrepetitive DNA. Moreover, this rate of CpG transition varies according to the number of repeats, their length, and their level of divergence from the ancestral sequence (up to 2.7 times higher in long, lowly divergent TEs compared with unique sequences). This observation strongly suggests the existence of a homology-dependent methylation (HDM) mechanism in mammalian genomes. We propose that HDM is a direct consequence of interfering RNA-induced transcriptional gene silencing.
Document type :
Journal articles
Complete list of metadata
Contributor : Stéphane Delmotte Connect in order to contact the contributor
Submitted on : Wednesday, October 28, 2009 - 5:12:38 PM
Last modification on : Sunday, September 25, 2022 - 3:55:15 AM

Links full text




J. Meunier, A. Khelifi, V. Navratil, L. Duret. Homology-dependent methylation in primate repetitive DNA.. Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2005, 102 (15), pp.5471-6. ⟨10.1073/pnas.0408986102⟩. ⟨hal-00427789⟩



Record views