Serotonin binds to purified neuronal nitric oxide synthase: a possible explanation for ROS production induced by 5HT in the presence of nNOS.

Abstract : Serotonin (5HT) was shown to induce in vitro the production of ROS in the presence of neuronal nitric oxide synthase (nNOS) in addition to the basal NO(+) formation. With the aim of understanding this mechanism, this study investigated the potential binding of 5HT to nNOS. By using [(3)H]5HT, it is reported here that 5HT binds to nNOS, but only when the enzyme is active and in a superoxide-dependent manner. This binding is prevented by DPI but not by L-NAME. The formation of 5HT-nNOS complex was shown to be very well correlated with the production of ROS by 5HT in the presence of nNOS. A mechanism involving nNOS only in its initial step is proposed to explain both the formation of 5HT-nNOS complex and the production of ROS observed in the presence of nNOS and 5HT.
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Submitted on : Thursday, June 18, 2009 - 10:16:35 AM
Last modification on : Tuesday, April 30, 2019 - 2:24:02 PM

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Maud Bréard, Catherine Grillon. Serotonin binds to purified neuronal nitric oxide synthase: a possible explanation for ROS production induced by 5HT in the presence of nNOS.. Free Radic Res, 2009, 43 (3), pp.206-13. ⟨10.1080/10715760802676662⟩. ⟨hal-00396425⟩

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