PNA-peptide conjugates as intracellular gene control agents.

Gabriela D Ivanova , Martin M Fabani , Andrey A Arzumanov (1) , Rachida Abes (2) , Haifang Yin , Bernard Lebleu (2) , Matthew Wood (3) , Michael J Gait

1 ISTC - Engelhardt Institute of Molecular Biology
2 DIMNP - Dynamique des interactions membranaires normales et pathologiques
3 HYSED (Hydrodynamics and Sedimentology Laboratory)

Serum-stabilized PNA-internalization peptides (Pip) conjugated to PNA complementary to the 705 aberrant beta-globin splice site are able to correct splicing and increase luciferase production in Hela pLuc705 cells with sub microM EC(50) in the absence of a transfection agent. Inhibition of microRNA-122 in liver cells is achieved by treatment with complementary PNA containing just a few attached Lys residues, again without need of a transfection agent.

Domaines: Biochimie, Biologie Moléculaire

Gabriela D Ivanova, Martin M Fabani, Andrey A Arzumanov, Rachida Abes, Haifang Yin, et al.. PNA-peptide conjugates as intracellular gene control agents.. Nucleic Acids Symposium Series, 2008, 52, pp.31-2. ⟨10.1093/nass/nrn016⟩. ⟨hal-00374442⟩