In vitro efficacy of newly designed vancomycin-based microparticles

Abstract : PURPOSE: To compare the in vitro bactericidal and anti-adhesion properties of vancomycin-based microparticles and lyophilized vancomycin and estimate their relevance to perioperative antibiotic prophylaxis and endophthalmitis prevention. SETTING: University research laboratory, Lyon, France. METHODS: The bactericidal and anti-adhesion properties of a newly designed drug-delivery system were assessed on Staphylococcus epidermidis clinical strain N890074 containing the intercellular adhesion locus ica. Lyophilized vancomycin at 20 μg/mL was used as a standard. The new drug-delivery system, designed for the study, consisted of sterile, biocompatible, and biodegradable microparticles with continuous release of vancomycin. To obtain bacterial killing and anti-adhesion curves, experiments were first performed in a bacterial suspension containing 1000 colony-forming units per milliliter. Experiments were then performed with intraocular lenses incubated in the suspension. Efficacy was investigated by bacterial counts and scanning electron microscopy observations. RESULTS: The bactericidal and anti-adhesion effects of vancomycin-based microparticles started after 3 hours (P<.002) and 1 hour (P<.001), respectively, and of lyophilized vancomycin, after 1 hour (P =.004) and 1 hour (P<.001), respectively. There was no difference between the 2 forms of vancomycin in the bactericidal effect starting at 21 hours and the anti-adhesion effect starting at 6 hours (P>.05). CONCLUSIONS: The newly designed vancomycin-based microparticles showed relevant antibacterial and anti-adhesion properties after releasing a sufficient antibacterial quantity, proving that vancomycin remains efficient after undergoing the encapsulation process.
Type de document :
Article dans une revue
Journal of Cataract & Refractive Surgery, 2007, 33 (4), pp.702-708
Domaine :
Liste complète des métadonnées
Contributeur : Hélène Lesur <>
Soumis le : jeudi 2 avril 2009 - 15:57:39
Dernière modification le : jeudi 7 février 2019 - 16:42:40


  • HAL Id : hal-00372861, version 1


Walid Hachicha, Hatem Fessi, Emmanuelle Casoli-Bergeron, Mi-Yeon Lee, Chiraz Jaafar, et al.. In vitro efficacy of newly designed vancomycin-based microparticles. Journal of Cataract & Refractive Surgery, 2007, 33 (4), pp.702-708. 〈hal-00372861〉



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