Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the gamma-decalactone production by Yarrowia lipolytica.

Yves Waché (1, 2, 3) , K. Bergmark , J. L. Courthaudon , M. Aguedo (2) , J. M. Nicaud (4, 5) , J. M. Belin (2, 3)

1 Microbiologie
2 GPMA - Génie des Procédés Microbiologiques et Alimentaires
3 Laboratoire de Microbiologie
4 MGM - Microbiologie et Génétique Moléculaire
5 Collection de Levures d'Intérêt Biotechnologique et Laboratoire de Génétique Moléculaire et Cellulaire

Size of methyl ricinoleate droplets during biotransformation into gamma-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d32 of 2.5 microm whereas it was 0.7 microm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 microm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions.

Mots clés: Culture Media; Lactones; Microscopy; Particle Size; Ricinoleic Acids; Time Factors; Yeasts

Mots clés: Biotransformation; Confocal

Domaines: Biotechnologie; Biotechnologies; Biochimie, Biologie Moléculaire

Yves Waché, K. Bergmark, J. L. Courthaudon, M. Aguedo, J. M. Nicaud, et al.. Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the gamma-decalactone production by Yarrowia lipolytica.. Letters in Applied Microbiology, 2000, 30 (3), pp.183-7. ⟨hal-00368235⟩