Detection of Extracellular Phosphatase Activity at the Single-Cell Level by Enzyme-Labeled Fluorescence and Flow Cytometry : the Importance of Time Kinetics in ELFA Labeling

Solange Duhamel; Gérald Grégori; France van Wambeke; Jiri Nedoma

doi:10.1002/cyto.a.20686

Journal articles

Detection of Extracellular Phosphatase Activity at the Single-Cell Level by Enzyme-Labeled Fluorescence and Flow Cytometry : the Importance of Time Kinetics in ELFA Labeling

Solange Duhamel ^{1, 2} Gérald Grégori ¹ France van Wambeke ¹ Jiri Nedoma ³

1 LMGEM - Laboratoire de MicrobiologiE de Géochimie et d'Ecologie Marines

2 LOB - Laboratoire d'océanographie et de biogéochimie

3 Biological Centre of the Academy of Sciences

Keywords : ELF phosphate alkaline phosphatase heterotrophic bacteria kinetics

Document type :

Journal articles

Domain :

Sciences of the Universe [physics] / Ocean, Atmosphere

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https://hal.archives-ouvertes.fr/hal-00363524
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Submitted on : Monday, February 23, 2009 - 3:24:44 PM
Last modification on : Tuesday, October 19, 2021 - 6:59:06 PM

Detection of Extracellular Phosphatase Activity at the Single-Cell Level by Enzyme-Labeled Fluorescence and Flow Cytometry : the Importance of Time Kinetics in ELFA Labeling

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Detection of Extracellular Phosphatase Activity at the Single-Cell Level by Enzyme-Labeled Fluorescence and Flow Cytometry : the Importance of Time Kinetics in ELFA Labeling

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