Design of polypeptide-functionalized polystyrene microspheres

Abstract : In this contribution, the principle of spontaneous surface segregation has been applied for the preparation of polypeptide-functionalized polystyrene microspheres. For that purpose, an amphiphilic diblock copolymer was introduced in the mixture styrene/divinylbenzene and polymerized using AIBN as initiator. During the polymerization, cross-linked particles were obtained in which the diblock copolymer was encapsulated. The amphiphilic diblock copolymers used throughout this study contain a hydrophilic polypeptide segment, either poly(L-lysine) or poly(L-glutamic acid) and a hydrophobic polystyrene block. After 4 It of polymerization, rather monodisperse particles with sizes of similar to 3-4 mu m were obtained. Upon annealing in hot water, the hydrophilic polypeptides migrate to the interface, hence, either positively charged or neutral particles were obtained when poly(L-lysine) is revealed at the surface and exposed to acidic or basic pH, respectively. On the opposite, negatively charged particles were achieved in basic pH water by using poly(L-glutamic acid) as additive. The surface chemical composition was modified by changing the environment of the particles. Thus, exposure in toluene provoked a surface rearrangement, and due to its affinity, the polystyrene block reorients toward the interface.
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https://hal.archives-ouvertes.fr/hal-00340020
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Submitted on : Wednesday, November 19, 2008 - 4:18:02 PM
Last modification on : Thursday, January 11, 2018 - 6:28:09 AM

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Antoine Bousquet, Romain Perrier-Cornet, Emmanuel Ibarboure, Eric Papon, Christine Labrugère, et al.. Design of polypeptide-functionalized polystyrene microspheres. Biomacromolecules, American Chemical Society, 2008, 9 (7), pp.1811-1817. ⟨10.1021/bm8000292⟩. ⟨hal-00340020⟩

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