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Confocal imaging and tracking of the exocytotic routes for D-serine-mediated gliotransmission.

Abstract : D-Serine is an astrocyte-derived regulator for N-methyl-D-aspartate receptors, but the intracellular routes of its trafficking are still largely unknown. Here, we combined confocal microscopy with colocalization quantification to track the astrocytic organelles that store D-serine. We report that D-serine colocalizes with the transfected eGFP-synaptobrevin/VAMP2 and eGFP-cellubrevin/VAMP3, two v-SNAREs of the regulated secretory pathway. No significant colocalization was found with markers of the endosomal sorting and recycling system: EEA1, eGFP-endobrevin/VAMP8, eGFP-TI-VAMP/VAMP7, LAMP1, and CD63. Blockade of vesicular budding with colchicine shows that secretory vesicles import D-serine downstream to the Golgi apparatus. Finally, treatment of astrocytes with the Ca2+-ionophore A23187, glutamate agonists, or bradykinin trigger translocation of synaptobrevin/VAMP2 to the plasma membrane with a concomitant disappearance of D-serine from the regulated secretory pathway. Our results provide morphological evidence for a vesicular storage of D-serine in the regulated secretory pathway and the possible recruitment of these stores by Ca2+ mobilization to release D-serine.
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https://hal.archives-ouvertes.fr/hal-00332637
Contributor : Martine Bondidier <>
Submitted on : Tuesday, October 21, 2008 - 12:28:51 PM
Last modification on : Friday, April 10, 2020 - 5:22:12 PM

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Magalie Martineau, Thierry Galli, Gérard Baux, Jean-Pierre Mothet. Confocal imaging and tracking of the exocytotic routes for D-serine-mediated gliotransmission.. Glia, Wiley, 2008, 56 (12), pp.1271-84. ⟨10.1002/glia.20696⟩. ⟨hal-00332637⟩

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