The extracellular proteoglycan produced by Rhodella grisea

P. Capek (1) , Maria Matulova (2) , Bruno Combourieu (3)

1 Institute of Chemistry
2 Institute of Chemistry
3 SEESIB - Synthèse et étude de systèmes à intêret biologique

Highly viscous extracellular proteoglycan (EPG) has been isolated from culture medium of the unicellular red alga Rhodella grisea (Rhodophyceae) by ethanol precipitation. EPG was composed of xylose (29.3%), 3-O-methyl-xylose (26.0%), uronic acids (17.1%), rhamnose (14.4%), galactose (7.5%), glucose (3.9%), arabinose (1.4%) and mannose (0.4%), and traces of fucose, 4-O-methyl-xylose and 2,3-di-O-methyl-rhamnose or fucose. In addition, the polymer contained proteins (13.1%), sulphates and 13C-CP MAS spectra indicated the presence of acetyl and succinyl groups. The molecular mass was estimated to be 136,000. Ion-exchange chromatography afforded five fractions differing in composition of neutral sugars, uronic acids, and protein content indicating thus the complex structure of the EPG

Mots clés: Rhodella grisea; Methylated proteoglycan; Mass spectrometry; 13C-CP MAS NMR

Domaines: Biochimie, Biologie Moléculaire

P. Capek, Maria Matulova, Bruno Combourieu. The extracellular proteoglycan produced by Rhodella grisea. International Journal of Biological Macromolecules, 2008, 43, pp.390-393. ⟨hal-00325920⟩