In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy.

Stéphane Bancelin (1) , Carole Aimé (2) , Thibaud Coradin (2) , Marie-Claire Schanne-Klein (1)

1 LOB - Laboratoire d'optique et biosciences
2 MATBIO - Matériaux et Biologie

We implemented in situ time-lapse Second Harmonic Generation (SHG) microscopy to monitor the three-dimensional (3D) self-assembly of collagen in solution. As a proof of concept, we tuned the kinetics of fibril formation by varying the pH and measured the subsequent exponential increase of fibril volume density in SHG images. We obtained significantly different time constants at pH = 6.5 ± 0.3 and at pH = 7.5 ± 0.3. Moreover, we showed that we could focus on the growth of a single isolated collagen fibril because SHG microscopy is sensitive to well-organized fibrils with diameter below the optical resolution. This work illustrates the potential of SHG microscopy for the rational design and characterization of collagen-based biomaterials.

Domaines: Biomatériaux; Optique [physics.optics]; Imagerie

Stéphane Bancelin, Carole Aimé, Thibaud Coradin, Marie-Claire Schanne-Klein. In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy.. Biomedical optics express, 2012, 3 (6), pp.1446-54. ⟨10.1364/BOE.3.001446⟩. ⟨hal-00324324⟩