In vitro infection of human primary adipose cells with HIV-1: a reassessment.

S. Munier (1) , A. Borjabad (1) , M. Lemaire (1) , V. Mariot (1) , U. Hazan (1)

1 IBCP - Institut de biologie et chimie des protéines [Lyon]

We reassessed the infection ability of human primary preadipocytes. The use of X4, R5 or VSV-G-pseudotyped viral particles indicated that viral entry is the limiting step. However, transfection with HIV-1 receptors restored efficient infection. Analyses of CD4, CXCR4 and CCR5 expression on preadipocytes and adipocytes revealed that receptor co-expression levels did not permit HIV-1 entry into adipose cells from all biopsies tested. We concluded that adipose tissue cannot be infected with HIV-1 in vivo.We reassessed the infection ability of human primary preadipocytes. The use of X4, R5 or VSV-G-pseudotyped viral particles indicated that viral entry is the limiting step. However, transfection with HIV-1 receptors restored efficient infection. Analyses of CD4, CXCR4 and CCR5 expression on preadipocytes and adipocytes revealed that receptor co-expression levels did not permit HIV-1 entry into adipose cells from all biopsies tested. We concluded that adipose tissue cannot be infected with HIV-1 in vivo.

Domaines: Biochimie, Biologie Moléculaire

S. Munier, A. Borjabad, M. Lemaire, V. Mariot, U. Hazan. In vitro infection of human primary adipose cells with HIV-1: a reassessment.. AIDS. Official journal of the international AIDS Society, 2003, 17, pp.2537-2539. ⟨hal-00315040⟩