Immunogold labelling of feruloyl-arabinose of maize stem after rumen microbial degradation

C. Migne (1) , G. Prensier , J.P. Utille (2) , P. Angibeaud (2) , Agnes Cornu (1) , Elisabeth Grenet (1)

1 Station de recherches sur la nutrition des herbivores
2 CERMAV - Centre de Recherches sur les Macromolécules Végétales

Immunogold labelling of feruloyl-arabinose was performed on the base and the top of the apical internode of the stem of Co125 and W401 maize, harvested at anthesis +5 days, after incubation in the rumen in nylon bags for 4, 8 and 24h. The description of labelling evolution during rumen degradation was permitted by the quantification of the intensity of labelling in four tissues: sclerenchyma, fibres, xylem and parenchyma. The results are discussed in relation to maize digestibility.

Domaines: Alimentation et Nutrition

C. Migne, G. Prensier, J.P. Utille, P. Angibeaud, Agnes Cornu, et al.. Immunogold labelling of feruloyl-arabinose of maize stem after rumen microbial degradation. Journal of the Science of Food and Agriculture, 1999, 79 (9), pp.1163-1170. ⟨10.1002/(SICI)1097-0010(19990701)79:9<1163::AID-JSFA343>3.3.CO;2-W⟩. ⟨hal-00309777⟩