ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast
Résumé
An ICP-MS-assisted proteomics approach was developed for the characterization of Se-containing proteins in selenium-rich yeast. Ultrasound probe leaching of a yeast sample with 8 M urea and 4% CHAPS and purification were optimized for highest selenium and protein extraction yield. The proteins were separated by 2D gel electrophoresis. The spots containing selenium (14) were located by laser ablation-ICP-MS, excised and analysed by nanoHPLC with the consecutive ICP-MS and electrospray Q-TOF MS/MS detection. The method allowed a successful identification of 17 selenium-containing proteins. © The Royal Society of Chemistry 2008.