First evidence of the implication of nitric oxide in a mouse model for ciguatera fish poisoning

Abstract : The involvement of the nitric oxide (NO) pathway in ciguatera fish poisoning (CFP) has been investigated, in vitro and in vivo, in a ciguatoxin (CTX)/mouse model. The induction of inducible nitric oxide synthase (iNOS) synthesis at the mRNA level was kinetically measured using a real-time PCR protocol based on the LightCycler® technology. CTX-pulsed Neuro-2a cells (1 ng/mL) and peripheral blood mononuclear cells from CTXinjected mice (1ng/g), were demonstrated to express iNOS in a time-dependent manner. This strongly suggests that NO might be responsible for certain ciguatera symptoms (e.g. hypotension, allergenic effects and Chronic Fatigue Syndrome) which could not be solely explained by the activation of voltage-gated sodium channels. This hypothesis is supported by the observation that the most currently used drugs for the treatment of CFP are free radical scavengers. In conclusion, the implication of NO in CFP paves the way for new therapies for both occidental and traditional medicines, together with new CTXs detection and clinical diagnostic tools.
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Conference papers
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https://hal.archives-ouvertes.fr/hal-00291772
Contributor : Michèle Outters-Lafaye <>
Submitted on : Sunday, June 29, 2008 - 11:27:50 PM
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S. Pauillac, F. Vernel-Pauillac, S. Kumar-Roine, Martin-Pierre Sauviat, Evelyne Benoit, et al.. First evidence of the implication of nitric oxide in a mouse model for ciguatera fish poisoning. 12th International Conference on Harmful Algae, 4-8 September 2006, Sep 2006, Copenhagen, Denmark. pp.316-318. ⟨hal-00291772⟩

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