Human adipose tissue-derived multipotent stem cells differentiate in vitro and in vivo into osteocyte-like cells.

C. Elabd (1) , C. Chiellini (1) , A. Massoudi (1) , O. Cochet (1) , L.E. Zaragosi (1) , C. Trojani (2) , J.F. Michels (3) , P. Weiss (4) , G. Carle (2) , N. Rochet (2) , C.A. Dechesne (1) , G. Ailhaud (1) , C. Dani (1) , E.Z. Amri (1)

1 IBDC - Institut de signalisation, biologie du développement et cancer - Institute of Developmental Biology and Cancer
2 GéPITOS - Génétique, physiopathologie et ingénierie du tissu osseux
3 Service d' Anatomopathologie
4 LIOAD - Laboratoire d'ingénierie osteo-articulaire et dentaire

Cell-based therapies are used to treat bone defects. We recently described that human multipotent adipose-derived stem (hMADS) cells, which exhibit a normal karyotype, self renewal, and the maintenance of their differentiation properties, are able to differentiate into different lineages. Herein, we show that hMADS cells can differentiate into osteocyte-like cells. In the presence of a low amount of serum and EGF, hMADS cells express specific molecular markers, among which alkaline phosphatase, CBFA-1, osteocalcin, DMP1, PHEX, and podoplanin and develop functional gap-junctions. When loaded on a hardening injectable bone substitute (HIBS) biomaterial and injected subcutaneously into nude mice, hMADS cells develop mineralized woven bone 4 weeks after implantation. Thus hMADS cells represent a valuable tool for pharmacological and biological studies of osteoblast differentiation in vitro and bone development in vivo.

Domaines: Biochimie, Biologie Moléculaire; Biologie du développement

C. Elabd, C. Chiellini, A. Massoudi, O. Cochet, L.E. Zaragosi, et al.. Human adipose tissue-derived multipotent stem cells differentiate in vitro and in vivo into osteocyte-like cells.. Biochemical and Biophysical Research Communications, 2007, 361 (2), pp.342-8. ⟨10.1016/j.bbrc.2007.06.180⟩. ⟨hal-00282765⟩