Rapid coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip based mass spectrometry for the identification of proteins in nucleoprotein interactions.

Emeline Bouffartigues (1) , Hervé Leh (1, 2) , Marielle Anger-Leroy (3) , Sylvie Rimsky (1) , Malcolm Buckle (1)

1 LBPA - Laboratoire de Biologie et de Pharmacologie Appliquée
2 Bioalliancepharma
3 GenOptics SA Centre Scientifique

We compared coupling approaches of SPR to LC-MS and ProteinChip-based mass spectrometry (SELDI) as a means of identifying proteins captured on DNA surfaces. The approach we outline has the potential to allow multiple, quantitative analysis of macromolecular interactions followed by rapid mass spectrometry identification of retained material.

Domaines: Biochimie, Biologie Moléculaire

Emeline Bouffartigues, Hervé Leh, Marielle Anger-Leroy, Sylvie Rimsky, Malcolm Buckle. Rapid coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip based mass spectrometry for the identification of proteins in nucleoprotein interactions.. Nucleic Acids Research, 2007, 35 (6), pp.e39. ⟨10.1093/nar/gkm030⟩. ⟨hal-00277436⟩