Cells tracking in a live zebrafish embryo.

Camilo Melani (1) , Matteo Campana (1) , Benoit Lombardot (1) , Barbara Rizzi (1) , Federico Veronesi (1) , Cecilia Zanella (1) , Paul Bourgine , Karol Mikula (1) , Nadine Peyriéras (2, 3) , Alessandro Sarti (1)

1 DEIS - Dipartimento di Elettronica, Informatica e Sistemistica
2 DEPSN - Développement, évolution et plasticité du système nerveux
3 INAF - Institut de Neurobiologie Alfred Fessard

We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei.

Domaines: Neurosciences [q-bio.NC]

Camilo Melani, Matteo Campana, Benoit Lombardot, Barbara Rizzi, Federico Veronesi, et al.. Cells tracking in a live zebrafish embryo.. Conference proceedings : .. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2007, 2007, pp.1631-4. ⟨10.1109/IEMBS.2007.4352619⟩. ⟨hal-00275367⟩