Protein profiling of hemocytes from the terrestrial crustacean Armadillidium vulgare

J. Herbiniere (1) , P. Greve (1) , J.-M. Strub (2) , D. Thierse (2) , Frelon Raimond M. (1) , A. V. Dorsselaer (1) , G. Martin (3) , C. Braquart-Varnier (1)

1 LEES - Laboratoire Écologie, Évolution, Symbiose [UMR 6556 – Poitiers]
2 Substances naturelles/chimie moléculaire
3 GBPC - Génétique et biologie des populations de crustacés

To establish and maintain a successful infection, microbial pathogens have evolved various strategies to infect the host in the face of a functional immune system. In this context, the alpha-proteobacteria Wolbachia capacities to infect new host species have been greatly evidenced. Indeed, in terrestrial isopods, experimentally transferred Wolbachia invade all host tissues, including immune cells such as hemocytes. To investigate mechanisms that have to be avoided by bacteria to maintain themselves in hemocytes, we characterized the hemocyte proteome of Armadillidium vulgare by a 2D gel electrophoresis approach. Fifty-six proteins were identified and classified into functional groups (stress and immunity, glucose metabolisms, cytoskeleton, others). We focused on immune response and cytoskeleton proteins often exploited by bacteria to invade their host. From the microsequences obtained by mass spectrometry, PCR primers were designed to amplify seven partial cDNAs encoding masquerade, alpha2-macroglobulin, transglutaminase, MnSOD, calreticulin, cyclophilin, and vinculin, confirming their expression in hemocytes.

Domaines: Biodiversité et Ecologie; Génétique des populations [q-bio.PE]

J. Herbiniere, P. Greve, J.-M. Strub, D. Thierse, Frelon Raimond M., et al.. Protein profiling of hemocytes from the terrestrial crustacean Armadillidium vulgare. Developmental and Comparative Immunology, 2008, 32, pp.875-882. ⟨10.1016/j.dci.2008.01.007⟩. ⟨hal-00270694⟩