Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2007

Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI

Marcelo Gonzalez
  • Fonction : Auteur
Yannick Gueguen
Delphine Destoumieux-Garzón
Bernard Romestand
  • Fonction : Auteur
Julie Fievet
  • Fonction : Auteur
Jean-Michel Escoubas
Ofer Levy
  • Fonction : Auteur
Laure Sauné
  • Fonction : Auteur
Evelyne Bachère
  • Fonction : Auteur

Résumé

A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C. gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). Therefore, Cg-BPI, whose expression profile and biological properties are reminiscent of mammalian BPIs, may provide a first line of defense against potential bacterial invasion. To our knowledge, this is the first characterization of a BPI in an invertebrate.

Dates et versions

hal-00258926 , version 1 (26-02-2008)

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Marcelo Gonzalez, Yannick Gueguen, Delphine Destoumieux-Garzón, Bernard Romestand, Julie Fievet, et al.. Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104 (45), pp.17759-64. ⟨10.1073/pnas.0702281104⟩. ⟨hal-00258926⟩
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