Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours.

Atefeh Seghatoleslam , Alberto Zambrano , Regine Millon , Gitali Ganguli , Manuela Argentini (1) , Anne Cromer , Joseph Abecassis , Bohdan Wasylyk (1)

1 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

We have identified by differential display a number of novel genes that are expressed in hypopharyngeal head and neck squamous cell carcinoma. We report here the characterisation of one of these novel human genes, LOC92912, that encodes a protein of 375 amino acids. The protein contains a RWD domain, a coiled-coil, and an E2 ubiquitin conjugating enzyme domain. LOC92912 is upregulated in about 85% of tumour samples. It is expressed in tumour masses and in invasive epithelium, and is located in the cytoplasm of cells. To gain insights into its functions, we identified potential interacting partners by immunoaffinity purification of the flag tagged protein followed by MALDI peptide mass fingerprinting mass spectrometry. Actin and six actin-binding proteins were unambiguously identified as potential interacting partners, suggesting that LOC92912's functions may be linked with the cytoskeleton. This novel human gene may represent a new target for cancer therapeutics.

Domaines: Biologie moléculaire

Atefeh Seghatoleslam, Alberto Zambrano, Regine Millon, Gitali Ganguli, Manuela Argentini, et al.. Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours.. Biochemical and Biophysical Research Communications, 2006, 339 (1), pp.422-9. ⟨10.1016/j.bbrc.2005.11.026⟩. ⟨hal-00188137⟩